Our experience with enzyme-linked immunospot assay in the laboratory diagnostics of lyme borreliosis
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چکیده
منابع مشابه
Our experience with enzyme-linked immunospot assay in the laboratory diagnostics of lyme borreliosis
Background Lyme borreliosis (LB) is multisystem infectious disease with various clinical manifestations. In Europe, LB is caused by gram-negative spirochetal bacteria of the complex Borrelia burgdorferi sensu lato. The most important for human pathogenicity are B. garinii, B. afzelii and B.burgdorferi sensu stricto. Indirect laboratory diagnostics of LB is based on serological methods such as E...
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A novel antigen preparation--the synthetic C6 peptide, a conserved portion of the variable VlsE antigens of Borrelia burgdorferi--has been evaluated for serodiagnosis of Lyme borreliosis (LB) by an ELISA procedure. Serum specimens were from early and late LB patients, all resident in an endemic area in north-eastern Italy. The specificity of the test approached the 100% and sensitivity was in t...
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Class-specific enzyme-linked immunosorbent assays (ELISAs) with purified recombinant antigens of Borrelia burgdorferi sensu stricto and Western blot analyses with whole cells of this spirochete were used to test human sera to determine which antigens were diagnostically important. In analyses for immunoglobulin M (IgM) antibodies, 14 (82%) of 17 serum samples from persons who had erythema migra...
متن کاملCost-efficient quantification of enzyme-linked immunospot.
Enzyme-linked immunospot (ELISPOT) analysis is a sensitive technique for the detection and quantification of antigen-specific T cells (5,7) and also for the detection of various cytokines released by immune cells (2). It is used extensively to monitor peptide-specific T cell responses in vaccination protocols (3), melanoma patients (6), and adoptive cellular immunotherapy (9) because it can det...
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ژورنال
عنوان ژورنال: World Allergy Organization Journal
سال: 2015
ISSN: 1939-4551
DOI: 10.1186/1939-4551-8-s1-a121